Protein pyrophosphorylation: moving forward
نویسنده
چکیده
Genetic ablation of inositol pyrophosphate synthesis has established the fundamental importance of this class of molecules to the eukaryote cell. These studies, however, must be complemented by cell biology and biochemical approaches to appreciate the signalling involved in the processes regulated by inositol pyrophosphates. A recent study by Chanduri et al. published in the Biochemical Journal, by integrating multiple experimental approaches, demonstrated that inositol pyrophosphates regulate intracellular vesicular movement. In particular, the vesicular transport along the microtubule that is driven by the motor protein complex dynein. Importantly, one subunit of this cellular motor, dynein 1 intermediate chain 2, undergoes serine pyrophosphorylation, a post-translational modification driven by inositol pyrophosphates. The pyrophosphorylation status of this dynein intermediate chain regulates its interaction with dynactin, which recruits the motor to vesicles. This mechanistically might explain how inositol pyrophosphates control intracellular membrane trafficking. By dissecting the serine pyrophosphorylation process, this work increases our awareness of this modification, underappreciated by the scientific literature but probably not by the eukaryotic cell.
منابع مشابه
Correction: The new world of inorganic polyphosphates.
Figure 1 Model of two independent PTMs, serine-pyrophosphorylation and lysine-polyphosphorylation Protein pyrophosphorylation is the transfer of the b-phosphate group of the pyrophosphate moiety of IP7 on to a pre-phosphorylated serine residue (left) [16,17]. Conversely, protein polyphosphorylation is the covalent attachment of a chain of inorganic polyP on to a lysine residue dependent on the ...
متن کاملProtein pyrophosphorylation by inositol pyrophosphates is a posttranslational event.
In a previous study, we showed that the inositol pyrophosphate diphosphoinositol pentakisphosphate (IP(7)) physiologically phosphorylates mammalian and yeast proteins. We now report that this phosphate transfer reflects pyrophosphorylation. Thus, proteins must be prephosphorylated by ATP to prime them for IP(7) phosphorylation. IP(7) phosphorylates synthetic phosphopeptides but not if their pho...
متن کاملDefining Integrated Knowledge Translation and Moving Forward: A Response to Recent Commentaries
متن کامل
Inositol hexakisphosphate kinase 1 (IP6K1) activity is required for cytoplasmic dynein-driven transport
Inositol pyrophosphates, such as diphosphoinositol pentakisphosphate (IP7), are conserved eukaryotic signaling molecules that possess pyrophosphate and monophosphate moieties. Generated predominantly by inositol hexakisphosphate kinases (IP6Ks), inositol pyrophosphates can modulate protein function by posttranslational serine pyrophosphorylation. Here, we report inositol pyrophosphates as novel...
متن کاملVibration Suppression of Simply Supported Beam under a Moving Mass using On-Line Neural Network Controller
In this paper, model reference neural network structure is used as a controller for vibration suppression of the Euler–Bernoulli beam under the excitation of moving mass travelling along a vibrating path. The non-dimensional equation of motion the beam acted upon by a moving mass is achieved. A Dirac-delta function is used to describe the position of the moving mass along the beam and its iner...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره 473 شماره
صفحات -
تاریخ انتشار 2016